As you are probably aware, antisense oligonucleotides are synthetic single-stranded chains of nucleic acids that bind to RNA (ribonucleic acid) ; and in doing so alter the or reduce the expression of the target RNA. They reduce expression of mutant proteins and restore expression of wild type proteins. Anti-sense oligonucleotides also are known to interfere with pre-mRNA splicing. In eukaryotic cells, the double-stranded DNA templates are housed in the nucleus of the cell. The twenty or so oncogenes (which are genes that are found in human DNA which can predispose a human being to various types of cancer) are flanked by five- prime and three-prime DNA sequences which render the the oncogene genome unable to express cancer-causing genes. However, if a frame-shift or some other type of mutation occurs, the messenger RNA that is transcribed from one of the template strands of DNA in the nucleus may intermediate the translation of cancer causing proteins (as this mRNA will find it’s way to the rough endoplasmic reticulum and be read by the anti-codons of tRNA), causing sequential amino acids to form proteins by peptide linkages and ultimately lead to a cancerous condition.